Veterinarnaya Klinicheskaya Patologiya M Dzhekson3661431

It is known that cadmium has the ability to supersede biometals from the protein structures, inhibits the catalytic properties of the enzymes, depresses innate immunity factors, exacerbates the infectious process. The aim of this work was to explore the dynamics of the specific antibodies synthesis in M.bovis infected rabbits and the impact of intoxication with low doses of cadmium chloride on it. Materials and methods. The animals were divided into 4 groups, three rabbits each. The chronic intoxication was caused by using cadmium chloride the aqueous solution of which was administered orally to rabbits of groups 1 and 3 daily for 60 days in a dose of 1.5 mg/kg. The animals of groups 2 and 4 received placebo. After 14 days from the beginning of the experiment rabbits of groups 2 and 3 were infected with M.bovis Bovinus-8.

The Mission and Vision of School-lyceum №6 “Today is a great day to begin something new” Our aim is: to open the doors of their minds to the world, through mutual understanding, trust and respect;.

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The antibody genesis dynamics was determined using indirect enzyme-linked immunosorbent assay (ELISA) based on home-made antigens and multiplex immunoassay. The diagnostic significance of the antigens was confirmed by analytical electrophoresis and immunoblot. The antibody response of infected animals (group 2) was detected since the 14th day after inoculation.Thus level of serum antibodies was stable until the end of the observation period (42 days), and the optical density reached 1.177 OD with M.bovis Bovinus-8 cell extract and 0.238 OD with their secondary metabolites.

There was a specific antibody synthesis delay of up to 28-42 days in rabbits infected after preliminary intoxication (group 3). ELISA results of uninfected animals (groups 1 and 4) did not exceed 0.179 OD and 0.078 OD for those antigens, respectively.

Using multiplex immunoassay it was established that the production of specific antibodies in M.bovis infected rabbits (group 2) was started on the 7th day, reaching the maximum values (greater than 50.000 RLU) on days 21-42.The evident suppression of antibody genesis observed in the group 3, when the maximum values did not exceed 9.000 RLU. The signals did not exceed the average background level (500 RLU) in rabbits of groups 1 and 4. It was found that cadmium chloride intoxication leads to inhibition of specific antibody production in M.bovis infected rabbits what was demonstrated by multiplex immunoassay and ELISA. Valeeva, A.R. Nekotoriye fiziologicheskiye i biokhimicheskiye pokazateli krolikov pri patogenezye tuberkulyoza na fonye intoksikatsii tayzhyolimi metalami [Some physiological and biochemical indices of rabbits in the pathogenesis of tuberculosis on a background of heavy metal intoxication]/ A.R.Valeeva, R.M.Akhmadeev, Z.Z.Aleeva// Aktualniye napravleniya innovatsionnogo razvitiya zhivotnovodstva i veterinarnoy meditsini: mat.Vseross.naych.-prakt. – Ufa:Bashkirskiy GAU, 2014.–P. Providing the population with dairy products depends on the development of agricultural enterprises with high profitability of milk production and increase of livestock herds and their and productivity.

It is known that cadmium has the ability to supersede biometals from the protein structures, inhibits the catalytic properties of the enzymes, depresses innate immunity factors, exacerbates the infectious process. The aim of this work was to explore the dynamics of the specific antibodies synthesis in M.bovis infected rabbits and the impact of intoxication with low doses of cadmium chloride on it. Materials and methods. The animals were divided into 4 groups, three rabbits each. The chronic intoxication was caused by using cadmium chloride the aqueous solution of which was administered orally to rabbits of groups 1 and 3 daily for 60 days in a dose of 1.5 mg/kg. The animals of groups 2 and 4 received placebo. After 14 days from the beginning of the experiment rabbits of groups 2 and 3 were infected with M.bovis Bovinus-8.

The Mission and Vision of School-lyceum №6 “Today is a great day to begin something new” Our aim is: to open the doors of their minds to the world, through mutual understanding, trust and respect;.

Autodesk provides students, educators, and institutions free access to 3ds Max software, in addition to learning tools. Get a free 3-year education license now. Maplesoft Maple 2015.1 (32-64Bit) Crack, Maplesoft Maple 2015.1 Windows 64bit Maplesim 2015.1 64bit - File Download - Rapid4me.com maplesim 2015.1 64bit:file search results 1 - 50 of 10000. Autodesk 3ds max design 2015 x64 rusifikator. Download 3ds Max Design 2015 SP2 3dsMaxDesign2015_SP2. This Service Pack contains recent fixes for Autodesk® 3ds Max Design 2015 software. It is strongly recommended that you read the readme document before installing the software. Autodesk 3ds Max Design 2015 Service Pack 2. Products and versions covered. 3ds Max Design 2015. After AutoCAD 2018 is installed, download and install an AutoCAD 2018 language pack to run AutoCAD in your preferred language. For use with AutoCAD 2018, and the AutoCAD software included in the 2018 Autodesk Design Suites – AutoCAD Design Suite, Product Design Suite, Factory Design Suite, Building Design Suite, Infrastructure Design Suite and Plant Design Suite.

The antibody genesis dynamics was determined using indirect enzyme-linked immunosorbent assay (ELISA) based on home-made antigens and multiplex immunoassay. The diagnostic significance of the antigens was confirmed by analytical electrophoresis and immunoblot. The antibody response of infected animals (group 2) was detected since the 14th day after inoculation.Thus level of serum antibodies was stable until the end of the observation period (42 days), and the optical density reached 1.177 OD with M.bovis Bovinus-8 cell extract and 0.238 OD with their secondary metabolites.

There was a specific antibody synthesis delay of up to 28-42 days in rabbits infected after preliminary intoxication (group 3). ELISA results of uninfected animals (groups 1 and 4) did not exceed 0.179 OD and 0.078 OD for those antigens, respectively.

Using multiplex immunoassay it was established that the production of specific antibodies in M.bovis infected rabbits (group 2) was started on the 7th day, reaching the maximum values (greater than 50.000 RLU) on days 21-42.The evident suppression of antibody genesis observed in the group 3, when the maximum values did not exceed 9.000 RLU. The signals did not exceed the average background level (500 RLU) in rabbits of groups 1 and 4. It was found that cadmium chloride intoxication leads to inhibition of specific antibody production in M.bovis infected rabbits what was demonstrated by multiplex immunoassay and ELISA. Valeeva, A.R. Nekotoriye fiziologicheskiye i biokhimicheskiye pokazateli krolikov pri patogenezye tuberkulyoza na fonye intoksikatsii tayzhyolimi metalami [Some physiological and biochemical indices of rabbits in the pathogenesis of tuberculosis on a background of heavy metal intoxication]/ A.R.Valeeva, R.M.Akhmadeev, Z.Z.Aleeva// Aktualniye napravleniya innovatsionnogo razvitiya zhivotnovodstva i veterinarnoy meditsini: mat.Vseross.naych.-prakt. – Ufa:Bashkirskiy GAU, 2014.–P. Providing the population with dairy products depends on the development of agricultural enterprises with high profitability of milk production and increase of livestock herds and their and productivity.